Displacement of Pathogens by an Engineered Bacterium Is a Multifactorial Process That Depends on Attachment Competition and Interspecific Antagonism

Al-Saedi, Fitua, Stones, Daniel H ORCID: 0000-0002-8981-7943, Vaz, Diana Pereira, Krachler, Anne Marie and Payne, S. M. (2016) Displacement of Pathogens by an Engineered Bacterium Is a Multifactorial Process That Depends on Attachment Competition and Interspecific Antagonism. Infection and Immunity, 84 (6). pp. 1704-1711. doi:10.1128/IAI.00020-16

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Abstract

Pathogen attachment to host cells is a key process during infection, and inhibition of pathogen adhesion is a promising approach to the prevention of infectious disease. We have previously shown that multivalent adhesion molecules (MAMs) are abundant in both pathogenic and commensal bacterial species, mediate early attachment to host cells, and can contribute to virulence. Here, we investigated the efficacy of an engineered bacterium expressing a commensal MAM on its surface in preventing pathogen attachment and pathogen-mediated cytotoxicity in a tissue culture infection model. We were able to dissect the individual contributions of adhesion and interspecific antagonism on the overall outcome of infection for a range of different pathogens by comparison with the results obtained with a fully synthetic adhesion inhibitor. We found that the potential of the engineered bacterium to outcompete the pathogen is not always solely dependent on its ability to hinder host attachment but, depending on the pathogenic species, may also include elements of interspecific antagonism, such as competition for nutrients and its ability to cause a loss of fitness due to production of antimicrobial factors.

Item Type:	Article
Article Type:	Article
Subjects:	Q Science > QR Microbiology
Divisions:	Schools and Research Institutes > School of Education and Science
Research Priority Areas:	Place, Environment and Community
Depositing User:	Daniel Stones
Date Deposited:	09 Oct 2018 10:33
Last Modified:	01 Sep 2023 15:37
URI:	https://eprints.glos.ac.uk/id/eprint/6065

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