Antibiotic-Producing Bacteria Collected from Seawater

Dashti, Khadijah M, Vali, Leila ORCID: 0009-0000-7740-3703, Almaraghi, Norya, Ebrahim, Hussain, Abul, Hassan and Dashti, Ali A (2024) Antibiotic-Producing Bacteria Collected from Seawater. Microbiology Research, 15 (4). pp. 2381-2393. doi:10.3390/microbiolres15040160

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Abstract

Background: Microorganisms are a known source of antibiotics. The study aimed to identify and screen antibiotic-producing microbes isolated from seawater. Method: Three of the fifty (50) bacteria isolated from seawater showed positive for antibiotic activity. The antimicrobial activity of Pseudomonas guguanensis (KD1) was screened against the ESKAPE pathogens using agarwell diffusion assays. P. guguanensis (KD1) was selected for the fermentation and extraction of antimicrobial compounds using solvent extraction assays. Results: P. guguanensis (KD1) produced the highest antibacterial activity after 36 h of cultivation, inhibiting S. aureus, E. faecium, A. baumannii and E. cloacae. According to sensitization assay, K. pneumoniae was impermeable to all the cell-free supernatants of P. guguanensis (KD1). Using agar-well diffusion assays, ethyl acetate extracts from the supernatant recorded zones of inhibition against S. aureus, E. faecium, and E. cloacae, producing zones of 20.1 ± 0.432, 17.8 ± 0.121 and 16 ± 0.162 mm, respectively. Acetonitrile extract from the supernatant inhibited A. baumannii and S. aureus, forming zones of inhibition 18.2 ± 0.323 mm and 18 ± 0.234. The minimum inhibitory concentration and minimum bactericidal concentration recorded for the ethyl acetate extract and acetonitrile extract ranged from 1.56 to 6.25 mg/mL and 12.5–25 mg/mL, respectively. Conclusions: P. guguanensis (KD1) offers a potential source of antibiotics for infections caused by multidrug-resistant bacteria.

Item Type:	Article
Article Type:	Article
Uncontrolled Keywords:	Pseudomonas; Antibiotic; Natural products; Antibiotic resistance; Antibacterial
Subjects:	G Geography. Anthropology. Recreation > GC Oceanography Q Science > Q Science (General) Q Science > QR Microbiology R Medicine > R Medicine (General) R Medicine > RM Therapeutics. Pharmacology
Divisions:	Schools and Research Institutes > School of Education and Science
Depositing User:	Anna Kerr
Date Deposited:	26 Nov 2024 09:55
Last Modified:	26 Nov 2024 10:00
URI:	https://eprints.glos.ac.uk/id/eprint/14586

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