Capillary Cortisol Sampling during High-Intensity Exercise

Fryer, Simon M ORCID: 0000-0003-0376-0104, Hillier, Stephen, Dickson, Tabitha, Draper, Nick, Stoner, Lee, Winter, David M, Young, John R and Cohen, L (2012) Capillary Cortisol Sampling during High-Intensity Exercise. International Journal of Sports Medicine, 33 (10). pp. 842-845. doi:10.1055/s-0032-1311584

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Abstract

Venepuncture is the established "gold standard" for sampling cortisol, but it is expensive, highly invasive and impractical for many experimental and clinical settings. Salivary free cortisol is a non-invasive and practical alternative; however, when cortisol concentrations exceed 500 nmol · L there is a lack of agreement between salivary (free) and venous (bound) cortisol. No known research has assessed whether capillary cortisol accurately reflects venous blood cortisol across a range of concentrations. The objective of the current study was to determine the agreement between capillary and venous blood samples of total plasma cortisol across a range of concentrations. 11 healthy male subjects (26.1±5.3 years) were recruited. Capillary and venous blood samples were collected pre and post (immediately post and post 5, 10, 15 and 20 min) a treadmill VO2max test. Regression analysis revealed a strong relationship (R2=0.96, y=1.0028x+1.2964 (P<0.05)) between capillary and venous cortisol concentrations. A Bland-Altman plot showed all data was within the upper and lower bounds of the 95% confidence interval, and no systematic bias was evident. In conclusion, capillary sampling is a valid technique for measuring bound cortisol across a range of concentrations.

Item Type:	Article
Article Type:	Article
Subjects:	G Geography. Anthropology. Recreation > GV Recreation Leisure > GV557 Sports R Medicine > RC Internal medicine > RC1200 Sports Medicine
Divisions:	Schools and Research Institutes > School of Education and Science
Research Priority Areas:	Health, Life Sciences, Sport and Wellbeing
Depositing User:	Anne Pengelly
Date Deposited:	20 Jul 2015 14:28
Last Modified:	31 Aug 2023 09:10
URI:	https://eprints.glos.ac.uk/id/eprint/2474

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