Tools
Tools
Lu, Wei-Ting, Hawley, Ben R, Skalka, George L, Baldock, Robert A 
ORCID: 0000-0002-4649-2966, Smith, Ewan M, Bader, Aldo S, Malewicz, Michal, Watts, Felicity Z, Wilczynska, Ania and Bushell, Martin
(2018)
Drosha drives the formation of DNA:RNA hybrids around DNA break sites to facilitate DNA repair.
Nature Communications, 9 (1).
Art 532.
doi:10.1038/s41467-018-02893-x
|
Text (Published version)
9779-Baldock-(2018)-Drosha-drives-the-formation-of-DNARNA-hybrids.pdf - Published Version Available under License Creative Commons Attribution 4.0. Download (2MB) | Preview |
Abstract
The error-free and efficient repair of DNA double-stranded breaks (DSBs) is extremely important for cell survival. RNA has been implicated in the resolution of DNA damage but the mechanism remains poorly understood. Here, we show that miRNA biogenesis enzymes, Drosha and Dicer, control the recruitment of repair factors from multiple pathways to sites of damage. Depletion of Drosha significantly reduces DNA repair by both homologous recombination (HR) and non-homologous end joining (NHEJ). Drosha is required within minutes of break induction, suggesting a central and early role for RNA processing in DNA repair. Sequencing of DNA:RNA hybrids reveals RNA invasion around DNA break sites in a Drosha-dependent manner. Removal of the RNA component of these structures results in impaired repair. These results show how RNA can be a direct and critical mediator of DNA damage repair in human cells.
| Item Type: | Article |
|---|---|
| Article Type: | Article |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH426 Genetics |
| Divisions: | Schools and Research Institutes > School of Education and Science |
| Research Priority Areas: | Place, Environment and Community |
| Depositing User: | Robbie Baldock |
| Date Deposited: | 08 Jun 2021 11:00 |
| Last Modified: | 31 Aug 2023 08:58 |
| URI: | https://eprints.glos.ac.uk/id/eprint/9779 |
University Staff: Request a correction | Repository Editors: Update this record
