Use of confidence radii to visualise significant differences in principal components analysis: Application to mammal assemblages at locations with different disturbance levels

Catlin-Groves, Christina L, Kirkhope, Claire L, Goodenough, Anne E ORCID: 0000-0002-7662-6670 and Stafford, Richard (2009) Use of confidence radii to visualise significant differences in principal components analysis: Application to mammal assemblages at locations with different disturbance levels. Ecological Informatics, 4 (3). pp. 147-151. doi:10.1016/j.ecoinf.2009.06.001

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Abstract

Multivariate statistical analysis is a powerful method of examining complex datasets, such as species assemblages, that does not suffer from the oversimplification prevalent in many univariate analyses. However, identifying whether data points on a multivariate plot are clustered is subjective, as there is no determination of significant differences between the points and no indication of the level of confidence in those points. The validity of drawing such conclusions may therefore be considered suspect. This paper describes a method of bootstrapping calculated principal components to estimate a confidence radius, similar to confidence intervals in univariate techniques. Plotting 3D scatterplots of the principal components, with the size of the spherical point representative of the level of confidence of the estimate, gives a clear and visual indication of significant difference between the points — where the spheres overlap there is no significant difference. We apply the technique to mammal assemblages at sites in Epping Forest (Essex, UK) that differ in the level of disturbance present and find that differences between some sites that appear large using traditional principal components analysis are actually not significantly different at the 95% confidence level, while other sites do differ significantly. Sites that differ most in anthropogenic disturbance are not significantly different in terms of assemblage structure.

Item Type: Article
Article Type: Article
Additional Information: Principal components analysis; Bootstrapping; Confidence intervals; Mammals; Community assemblage; Disturbance
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Schools and Research Institutes > School of Education and Science
Research Priority Areas: Place, Environment and Community
Depositing User: Anne Goodenough
Date Deposited: 01 Apr 2016 13:47
Last Modified: 31 Aug 2023 08:59
URI: https://eprints.glos.ac.uk/id/eprint/3329

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