EPLIN, a Putative Tumour Suppressor in Colorectal Cancer, Implications in Drug Resistance

Zeng, Jianyuan, Sanders, Andrew J ORCID: 0000-0002-7997-5286, Ye, Lin, Hargest, Rachel, Ruge, Fiona and Jiang, Wen G. (2022) EPLIN, a Putative Tumour Suppressor in Colorectal Cancer, Implications in Drug Resistance. International Journal of Molecular Sciences, 23. Art 15232. doi:10.3390/ijms232315232

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Abstract

Colorectal cancer is a serious threat to human health. Poor prognosis and frequently reported drug resistance urges research into novel biomarkers and mechanisms to aid in the understanding of the development and progression of colorectal cancer and to optimise therapeutic strategies. In the current study, we investigated the roles of a putative tumour suppressor, EPLIN, in colorectal cancer. Our clinical colorectal cancer cohort and online databases revealed a downregulation of EPLIN in colorectal cancer tissues compared with normal tissues. The reduced expression of EPLIN was associated with poor clinical outcomes of patients. In vitro cellular function assays showed that EPLIN elicited an inhibitory effect on cellular growth, adhesion, migration and invasion. Utilising a protein microarray on protein samples from normal and tumour patient tissues suggested HSP60, Her2 and other signalling events were novel potential interacting partners of EPLIN. It was further revealed that EPLIN and HSP60 were negative regulators of Her2 in colorectal cancer cells. The clinical cohort also demonstrated that expression of HSP60 and Her2 affected clinical outcomes, but most interestingly the combination of EPLIN, HSP60 and Her2 was able to identify patients with the most unfavourable clinical outcome by independently predicting patient overall survival and disease free survival. Furthermore, EPLIN and HSP60 exhibited potential to regulate cellular response to chemotherapeutic and EGFR/Her2 targeted therapeutic agents. In conclusion, EPLIN is an important prognostic factor for patients with colon cancer and reduced EPLIN in CRC contributes to aggressive traits of CRC cells and their responses to chemotherapeutic drugs. Collectively, EPLIN is a pivotal factor for the development and progression of colorectal cancer and has important clinical and therapeutic values in this cancer type.

Item Type:	Article
Article Type:	Article
Uncontrolled Keywords:	EPLIN; Colorectal cancer; HSP60; Her2; Drug resistance
Subjects:	R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer) R Medicine > RM Therapeutics. Pharmacology > RM695 Physical medicine. physical therapy including massage, exercise, occupational therapy, hydrotherapy, phototherapy, radiotherapy, thermotherapy, electrotherapy
Divisions:	Schools and Research Institutes > School of Education and Science
Research Priority Areas:	Place, Environment and Community
Depositing User:	Susan Turner
Date Deposited:	13 Dec 2022 15:00
Last Modified:	31 Aug 2023 08:57
URI:	https://eprints.glos.ac.uk/id/eprint/12057

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