Bacterial adhesion inhibitor prevents infection in a rodent surgical incision model

Huebinger, Ryan M., Do, D. H., Carlson, Deborah L., Yao, X., Stones, Daniel H ORCID: 0000-0002-8981-7943, de Souza Santos, Marcela, Vaz, Diana Pereira, Keen, E., Wolf, Steven E., Minei, J. P., Francis, K. P., Orth, Kim and Krachler, Anne Marie (2020) Bacterial adhesion inhibitor prevents infection in a rodent surgical incision model. Virulence, 11 (01). pp. 695-706. doi:10.1080/21505594.2020.1772652

[img]
Preview
Text (Published version)
8455-Stones-(2020)-Bacterial-adhesion-inhibitor-prevents-infection-in-a-rodent.pdf - Published Version
Available under License Creative Commons Attribution 4.0.

Download (6MB) | Preview

Abstract

Surgical site infection risk continues to increase due to lack of efficacy in current standard of care drugs. New methods to treat or prevent antibiotic-resistant bacterial infections are needed. Multivalent Adhesion Molecules (MAM) are bacterial adhesins required for virulence. We developed a bacterial adhesion inhibitor using recombinant MAM fragment bound to polymer scaffold, mimicking MAM7 display on the bacterial surface. Here, we test MAM7 inhibitor efficacy to prevent Gram-positive and Gram-negative infections. Using a rodent model of surgical infection, incision sites were infected with antibiotic-resistant bioluminescent strains of Staphylococcus aureus or Pseudomonas aeruginosa. Infections were treated with MAM7 inhibitor or control suspension. Bacterial abundance was quantified for nine days post infection. Inflammatory responses and histology were characterized using fixed tissue sections. MAM7 inhibitor treatment decreased burden of S. aureus and P. aeruginosa below detection threshold. Bacterial load of groups treated with control were significantly higher than MAM7 inhibitor-treated groups. Treatment with inhibitor reduced colonization of clinically-relevant pathogens in an in vivo model of surgical infection. Use of MAM7 inhibitor to block initial adhesion of bacteria to tissue in surgical incisions may reduce infection rates, presenting a strategy to mitigate overuse of antibiotics to prevent surgical site infections.

Item Type: Article
Article Type: Article
Uncontrolled Keywords: Surgical infection; Incision; Laceration; Bacterial adhesion; Anti-virulence; Pseudomonas; Staphylococcus
Subjects: Q Science > QR Microbiology
R Medicine > RD Surgery
R Medicine > RS Pharmacy and materia medica
Divisions: Schools and Research Institutes > School of Natural & Social Sciences
Research Priority Areas: Environmental Dynamics & Governance
Depositing User: Rhiannon Goodland
Date Deposited: 11 Jun 2020 09:47
Last Modified: 30 Jul 2020 12:37
URI: http://eprints.glos.ac.uk/id/eprint/8455

University Staff: Request a correction | Repository Editors: Update this record

University Of Gloucestershire

Bookmark and Share

Find Us On Social Media:

Social Media Icons Facebook Twitter Google+ YouTube Pinterest Linkedin

Other University Web Sites

University of Gloucestershire, The Park, Cheltenham, Gloucestershire, GL50 2RH. Telephone +44 (0)844 8010001.