Targeting bacterial adherence inhibits multidrug-resistant Pseudomonas aeruginosa infection following burn injury

Huebinger, Ryan M., Stones, Daniel H ORCID: 0000-0002-8981-7943, de Souza Santos, Marcela, Carlson, Deborah L., Song, Juquan, Vaz, Diana Pereira, Keen, Emma, Wolf, Steven E., Orth, Kim and Krachler, Anne Marie (2016) Targeting bacterial adherence inhibits multidrug-resistant Pseudomonas aeruginosa infection following burn injury. Scientific Reports, 6 (1). ISSN 2045-2322

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Abstract

Classical antimicrobial drugs target proliferation and therefore place microbes under extreme selective pressure to evolve resistance. Alternative drugs that target bacterial virulence without impacting survival directly offer an attractive solution to this problem, but to date few such molecules have been discovered. We previously discovered a widespread group of bacterial adhesins, termed Multivalent Adhesion Molecules (MAMs) that are essential for initial binding of bacteria to host tissues and virulence. Thus, targeting MAM-based adherence is a promising strategy for displacing pathogens from host tissues and inhibiting infection. Here, we show that topical application of polymeric microbeads functionalized with the adhesin MAM7 to a burn infected with multidrug-resistant Pseudomonas aeruginosa substantially decreased bacterial loads in the wound and prevented the spread of the infection into adjacent tissues. As a consequence, the application of this adhesion inhibitor allowed for vascularization and wound healing, and maintained local and systemic inflammatory responses to the burn. We propose that MAM7-functionalized microbeads can be used as a topical treatment, to reduce bacterial attachment and hence prevent bacterial colonization and infection of wounds. As adhesion is not required for microbial survival, this anti-infective strategy has the potential to treat multidrug-resistant infections and limit the emergence of drug-resistant pathogens.

Item Type: Article
Article Type: Article
Subjects: Q Science > QR Microbiology
Divisions: Schools and Research Institutes > School of Natural & Social Sciences
Research Priority Areas: Environmental Dynamics & Governance
Depositing User: Daniel Stones
Date Deposited: 09 Oct 2018 10:54
Last Modified: 11 Oct 2018 04:18
URI: http://eprints.glos.ac.uk/id/eprint/6067

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