Biomimetic Materials to Characterize Bacteria-host Interactions

Stones, Daniel H ORCID: 0000-0002-8981-7943, Al-Saedi, Fitua, Vaz, Diana, Perez-Soto, Nicolas and Krachler, Anne M. (2015) Biomimetic Materials to Characterize Bacteria-host Interactions. Journal of Visualized Experiments (105). ISSN 1940-087X

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Abstract

Bacterial attachment to host cells is one of the earliest events during bacterial colonization of host tissues and thus a key step during infection. The biochemical and functional characterization of adhesins mediating these initial bacteria-host interactions is often compromised by the presence of other bacterial factors, such as cell wall components or secreted molecules, which interfere with the analysis. This protocol describes the production and use of biomimetic materials, consisting of pure recombinant adhesins chemically coupled to commercially available, functionalized polystyrene beads, which have been used successfully to dissect the biochemical and functional interactions between individual bacterial adhesins and host cell receptors. Protocols for different coupling chemistries, allowing directional immobilization of recombinant adhesins on polymer scaffolds, and for assessment of the coupling efficiency of the resulting “bacteriomimetic” materials are also discussed. We further describe how these materials can be used as a tool to inhibit pathogen mediated cytotoxicity and discuss scope, limitations and further applications of this approach in studying bacterial - host interactions.

Item Type: Article
Article Type: Article
Additional Information: Stones, D. H., Al-Saedi, F., Vaz, D., Perez-Soto, N., Krachler, A. M. Biomimetic Materials to Characterize Bacteria-host Interactions. J. Vis. Exp. (105), e53400, doi:10.3791/53400 (2015). Article © Journal of Visualized Experiments (JoVE)
Uncontrolled Keywords: Infection; Issue 105; Host-pathogen interaction; Bacterial adhesion; Host cell attachment; Adhesin; Biomimicry; Bioengineering; Chemical biology
Subjects: Q Science > QR Microbiology
Divisions: Schools and Research Institutes > School of Natural & Social Sciences
Research Priority Areas: Environmental Dynamics & Governance
Depositing User: Daniel Stones
Date Deposited: 09 Oct 2018 09:57
Last Modified: 11 Oct 2018 05:47
URI: http://eprints.glos.ac.uk/id/eprint/6064

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