Expression of the leukemic prognostic marker CD7 is linked to epigenetic modifications in chronic myeloid leukemia.

Rogers, Sally L and Zhao, Yun and Jiang, Xiaoyan and Eaves, Connie J and Mager, Dixie L and Rouhi, Arefeh (2010) Expression of the leukemic prognostic marker CD7 is linked to epigenetic modifications in chronic myeloid leukemia. Molecular Cancer, 9. p. 41. ISSN 1476-4598

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Abstract

BACKGROUND Expression levels of the cell surface glycoprotein, CD7, and the serine protease, elastase 2 (ELA2), in the leukemic cells of patients with chronic myeloid leukemia (CML) have been associated with clinical outcome. However, little is known about the mechanisms that underlie the variable expression of these genes in the leukemic cells. RESULTS To address this question, we compared the level of their expression with the DNA methylation and histone acetylation status of 5' sequences of both genes in leukemic cell lines and primitive (lin-CD34+) leukemic cells from chronic phase CML patients. DNA methylation of the ELA2 gene promoter did not correlate with its expression pattern in lin-CD34+ cells from chronic phase CML patient samples even though there was clear differential DNA methylation of this locus in ELA2-expressing and non-expressing cell lines. In contrast, we found a strong relation between CD7 expression and transcription-permissive chromatin modifications, both at the level of DNA methylation and histone acetylation with evidence of hypomethylation of the CD7 promoter region in the lin-CD34+ cells from CML patients with high CD7 expression. CONCLUSION These findings indicate a link between epigenetic modifications and CD7 expression in primitive CML cells.

Item Type: Article
Article Type: Article
Uncontrolled Keywords: REF2014 Submission
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Q Science > QR Microbiology > QR180 Immunology
Divisions: Faculty of Business, Computing and Applied Sciences > School of Natural & Social Sciences > Environmental Sciences
Research Priority Areas: Environmental Dynamics & Governance
Depositing User: Sally Rogers
Date Deposited: 10 Mar 2015 11:27
Last Modified: 26 Aug 2016 12:40
URI: http://eprints.glos.ac.uk/id/eprint/2002

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